Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression

被引：327

作者：

Cui, Yazhou ^{[1
]}

Luan, Jing ^{[1
]}

Li, Haiying ^{[1
]}

Zhou, Xiaoyan ^{[1
]}

Han, Jinxiang ^{[1
]}

机构：

[1] Minist Hlth, Shandong Acad Med Sci, Shandong Med Biotechnol Ctr, Key Lab Biotechnol Drugs, Jinan 250062, Peoples R China

来源：

FEBS LETTERS | 2016年 / 590卷 / 01期

基金：

中国国家自然科学基金;

关键词：

cell-to-cell communication; exosomes; microRNA; osteoblast differentiation; MESENCHYMAL STEM-CELLS; MEDIATED TRANSFER; MECHANISMS;

D O I：

10.1002/1873-3468.12024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mineralizing osteoblasts (MOBs) can release exosomes, although the functional significance remains unclear. In the present study, we demonstrate that exosomes derived from mineralizing pre-osteoblast MC3T3-E1 cells can promote bone marrow stromal cell (ST2) differentiation to osteoblasts. We reveal that MOB-derived exosomes significantly influence miRNA profiles in recipient ST2 cells, and these changes tend to activate the Wnt signaling pathway by inhibiting Axin1 expression and increasing -catenin expression. We also suggest that MOB derived-exosomes partly induce the variation in miRNA expression in recipient ST2 cells by exosomal miRNA transfer. These findings suggest an exosome-mediated mode of cell-to-cell communication in the osteogenic microenvironment, and also indicate the potential of MOB exosomes in bone tissue engineering.

引用

页码：185 / 192

页数：8

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