ERBB2;
invasion;
P13 ' kinase;
human breast cancer;
human mammary epithelial cells;
D O I:
10.1054/bjoc.1999.0979
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
Amplification and overexpression of ERBB-2 in human breast cancer is thought to play a significant role in the progression of the disease; however, its precise role in the aetiology of altered phenotypes associated with human breast cancer is unknown. We have previously shown that exogenous overexpression of ERBB-2 conferred growth factor independence on human mammary epithelial cells. In this study, we show that ERBB-2 overexpression also causes the cells to acquire other characteristics exhibited by human breast cancer cells, such as anchorage-independent growth and invasion capabilities. ERBB-induced invasion is dependent on fibronectin and correlates with the down-regulation of cell surface alpha 4 integrin. In addition ERBB-2 co-immunoprecipitates with focal adhesion kinase (FAK) in these cells. We have also shown, by use of exogenously expressed PTEN and by treatment with the PIS'-kinase inhibitor LY294002, that ERBB-2-induced invasion is dependent on the PI3'-kinase pathway; however, PTEN does not dephosphorylate FAK in these cells. (C) 2000 Cancer Research Campaign.