Role of pinoline and melatonin in stabilizing hepatic microsomal membranes against oxidative stress

被引:98
作者
García, JJ
Reiter, RJ
Pié, J
Ortiz, GG
Cabrera, J
Sáinz, RM
Acuña-Castroviejo, D
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA
[2] Univ Zaragoza, Dept Physiol & Pharmacol, E-50009 Zaragoza, Spain
[3] Univ Granada, Dept Physiol, E-18071 Granada, Spain
关键词
pinoline; melatonin; lipid peroxidation; membrane fluidity; microsome;
D O I
10.1023/A:1005425213253
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We investigated the influence of pinoline (0.01-1.5 mM) on microsomal membrane fluidity before and after rigidity was induced by oxidative stress. In addition, we tested the effect of pinoline in the presence of 1 mM melatonin. The fluidity in rat hepatic microsomes was monitored using fluorescence spectroscopy and it was compared to the inhibition of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) production as a reflection of lipid peroxidation. Below 0.6 mM, pinoline inhibited membrane rigidity in a manner parallel to its inhibitory effect on MDA + 4-HDA formation. At concentrations between 1-1.5 mM, pinoline was less effective in stabilizing microsomal membranes than was predicted from its inhibition of lipid peroxidation. The addition of 1 mM melatonin enhanced the membrane-stabilizing activity of pinoline (0.01-0.6 mM). This cooperative effect was not observed for concentrations of pinoline between 1-1.5 mM. When pinoline was tested without induced oxidative damage, 1-1.5 mM pinoline maintained membrane fluidity at the same level as that recorded after induced lipid peroxidation. The results suggest that pinoline may be another pineal molecule that prevents membrane rigidity mediated by lipid peroxidation and this ability is enhanced by melatonin.
引用
收藏
页码:609 / 616
页数:8
相关论文
共 46 条
[21]  
LANGER SZ, 1984, EUR J PHARMACOL, V102, P379, DOI 10.1016/0014-2999(84)90275-9
[22]   THE IMMUNONEUROENDOCRINE ROLE OF MELATONIN [J].
MAESTRONI, GJM .
JOURNAL OF PINEAL RESEARCH, 1993, 14 (01) :1-10
[23]   Evaluation of the antioxidant activity of melatonin in vitro [J].
Marshall, KA ;
Reiter, RJ ;
Poeggeler, B ;
Aruoma, OI ;
Halliwell, B .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 21 (03) :307-315
[24]   INTERACTION OF VITAMIN-E WITH SATURATED PHOSPHOLIPID-BILAYERS [J].
MASSEY, JB ;
SHE, HS ;
POWNALL, HJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 106 (03) :842-847
[25]   Reaction of melatonin and related indoles with hydroxyl radicals: EPR and spin trapping investigations [J].
Matuszak, Z ;
Reszka, KJ ;
Chignell, CF .
FREE RADICAL BIOLOGY AND MEDICINE, 1997, 23 (03) :367-372
[26]   DISTRIBUTION OF MELATONIN IN MAMMALIAN-TISSUES - THE RELATIVE IMPORTANCE OF NUCLEAR VERSUS CYTOSOLIC LOCALIZATION [J].
MENENDEZPELAEZ, A ;
REITER, RJ .
JOURNAL OF PINEAL RESEARCH, 1993, 15 (02) :59-69
[27]  
OHKI K, 1984, J NUTR SCI VITAMINOL, V30, P221, DOI 10.3177/jnsv.30.221
[28]   EFFECTS OF ALPHA-TOCOPHEROL ON THE LIPID-PEROXIDATION AND FLUIDITY OF PORCINE INTESTINAL BRUSH-BORDER MEMBRANES [J].
OHYASHIKI, T ;
USHIRO, H ;
MOHRI, T .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 858 (02) :294-300
[29]  
Pahkla R, 1996, METHOD FIND EXP CLIN, V18, P359
[30]   Comparison of the antioxidant activity of melatonin and pinoline in vitro [J].
Pähkla, R ;
Zilmer, M ;
Kullisaar, T ;
Rägo, L .
JOURNAL OF PINEAL RESEARCH, 1998, 24 (02) :96-101