Structure of the human retinoic acid receptor beta 1 gene

被引：23

作者：

Toulouse, A ^{[1
]}

Morin, J ^{[1
]}

Pelletier, M ^{[1
]}

Bradley, WEC ^{[1
]}

机构：

[1] UNIV MONTREAL,DEPT MED,MONTREAL,PQ H2L 4M1,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

retinoic acid receptor; gene structure; 5'-untranslated region;

D O I：

10.1016/S0167-4781(96)00126-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor beta 1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RAR beta 1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RAR beta 3 which is widely expressed in mouse tissues.

引用

页码：1 / 4

页数：4

共 10 条

[1]

Chambon Pierre, 1994, Seminars in Cell Biology, V5, P115, DOI 10.1006/scel.1994.1015

[2] DIFFERENTIAL EXPRESSION AND LIGAND REGULATION OF THE RETINOIC ACID RECEPTOR-ALPHA AND RECEPTOR-BETA GENES [J].