Intracellular trehalose improves the survival of cryopreserved mammalian cells

被引：417

作者：

Eroglu, A

Russo, MJ

Bieganski, R

Fowler, A

Cheley, S

Bayley, H

Toner, M ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Engn Med, Boston, MA 02114 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Surg Serv, Boston, MA 02114 USA

[3] Shriners Hosp Crippled Children, Boston, MA 02114 USA

[4] Texas A&M Univ, Dept Med Biochem & Genet, College Stn, TX 77843 USA

[5] Univ Massachusetts, Dept Mech Engn, N Dartmouth, MA 02747 USA

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 02期

关键词：

trehalose; alpha-toxin; cryopreservation; fibroblasts; keratinocytes;

D O I：

10.1038/72608

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

引用

页码：163 / 167

页数：5

共 23 条

[1] Building doors into cells [J].