Quantitative Photoelectrochemical Detection of Biotin Conjugated CdSe/ZnS Quantum Dots on the Avidin Immobilized ITO Electrodes

被引：20

作者：

Bas, Deniz ^{[1
]}

Boyaci, Ismail Hakki ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Food Engn, TR-06800 Ankara, Turkey

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 16期

关键词：

Quantum dots; Photoelectrochemistry; Photocurrent; Avidin; Biotin; Biosensors; SEMICONDUCTOR NANOCRYSTALS; SURFACE MODIFICATION; DNA TARGETS; NANOPARTICLES; GOLD; BIOSENSOR; SYSTEMS;

D O I：

10.1002/elan.200904619

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Photocurrent generation from CdSe/ZnS (core/shell) quantum dots (QDs) in a photoelectrochemical cell was proposed to perform a bioaffinity biosensor in this study. The photocurrent of QDs is reversible and methylene blue as an electron transfer mediator causes a four-fold increase in the photocurrent. We further present quantitative photoelectrochemical detection of biotin conjugated QDs on the avidin immobilized ITO electrodes. A linear calibration graph was obtained in the range of 4 and 18 nM of biotin conjugated QDs with a coefficient of determination of 0.997. Results imply that QDs can be successfully used as photoelectroactive labels for the photoelectrochemical biosensor systems.

引用

页码：1829 / 1834

页数：6

共 27 条

[1]

Nanocrystal-based light-emitting diodes utilizing high-efficiency nonradiative energy transfer for color conversion [J].

Achermann, Marc ;

Petruska, Melissa A. ;

Koleske, Daniel D. ;

Crawford, Mary H. ;

Klimov, Victor I. .

NANO LETTERS, 2006, 6 (07) :1396-1400

[2]

Recent developments in II-VI and III-VI semiconductors and their applications in solar cells [J].

Afzaal, M ;

O'Brien, P .

JOURNAL OF MATERIALS CHEMISTRY, 2006, 16 (17) :1597-1602

[3]

Single-bead immunoassays using magnetic microparticles and spectral-shifting quantum dots [J].

Agrawal, Amit ;

Sathe, Tushar ;

Nie, Shuming .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (10) :3778-3782

[4]

Novel electrochemical interfaces with a tunable kinetic barrier by self-assembling organically modified silica gel and gold nanoparticles [J].

Bharathi, S ;

Nogami, M ;

Ikeda, S .

LANGMUIR, 2001, 17 (01) :1-4

[5]

Self-organized GaN quantum wire UV lasers [J].

Choi, HJ ;

Johnson, JC ;

He, RR ;

Lee, SK ;

Kim, F ;

Pauzauskie, P ;

Goldberger, J ;

Saykally, RJ ;

Yang, PD .

JOURNAL OF PHYSICAL CHEMISTRY B, 2003, 107 (34) :8721-8725

[6]

Direct immunotluorescence of plant Microtubules based on semiconductor nanocrystals [J].

Eggenberger, K. ;

Merkulov, A. ;

Darbandi, M. ;

Nann, T. ;

Nick, P. .

BIOCONJUGATE CHEMISTRY, 2007, 18 (06) :1879-1886

[7]

Color-selective semiconductor nanocrystal laser [J].

Eisler, HJ ;

Sundar, VC ;

Bawendi, MG ;

Walsh, M ;

Smith, HI ;

Klimov, V .

APPLIED PHYSICS LETTERS, 2002, 80 (24) :4614-4616

[8]

Electrochemical control of the photocurrent direction in intercalated DNA/CdS nanoparticle systems [J].

Gill, R ;

Patolsky, F ;

Katz, E ;

Willner, I .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (29) :4554-4557

[9]

Detection of single bacterial pathogens with semiconductor quantum dots [J].

Hahn, MA ;

Tabb, JS ;

Krauss, TD .

ANALYTICAL CHEMISTRY, 2005, 77 (15) :4861-4869

[10]

Femtomolar electrochemical detection of DNA targets using metal sulfide nanoparticles [J].

Hansen, JA ;

Mukhopadhyay, R ;

Hansen, JO ;

Gothelf, KV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (12) :3860-3861

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