A genetically encoded, fluorescent indicator for cyclic AMP in living cells

被引：371

作者：

Zaccolo, M ^{[1
]}

De Giorgi, F

Cho, CY

Feng, LX

Knapp, T

Negulescu, PA

Taylor, SS

Tsien, RY

Pozzan, T

机构：

[1] Univ Padua, Dept Expt Biomed Sci, I-35121 Padua, Italy

[2] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Pharmacol 0647, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[5] Aurora Biosci Corp, San Diego, CA 92121 USA

来源：

NATURE CELL BIOLOGY | 2000年 / 2卷 / 01期

关键词：

D O I：

10.1038/71345

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells, By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of cAMP, This new methodology opens the way to the elucidation of the biochemistry of cAMP in vivo.

引用

页码：25 / 29

页数：5

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