Exploring the architecture of potassium channels using chimaeras to reveal signal transduction

被引：6

作者：

Chanda, B ^{[1
]}

Tiwari, JK ^{[1
]}

Varshney, A ^{[1
]}

Mathew, MK ^{[1
]}

机构：

[1] Natl Ctr Biol Sci, TIFR, Bangalore 560065, Karnataka, India

来源：

BIOSCIENCE REPORTS | 1999年 / 19卷 / 04期

关键词：

chimaera; voltage sensing; ion channel; ion selectivity;

D O I：

10.1023/A:1020550408733

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chimaeric channel, 4N/1, generated from two outwardly rectifying Ki channels by linking the N-terminal cytoplasmic domain of hKv1.4 with the transmembrane body of hKv1.4, functions as an inward rectifier. The operating range of the channel is shifted to hyperpolarizing potentials and it is inactivated at resting membrane potentials. Coexpression of a truncated form of hKv1.1 with the N-terminal domain of hKv1.4 results in the same physiology as the chimaera implying specific interactions between the two segments.

引用

页码：301 / 306

页数：6

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