EPR spin trapping of protein radicals

被引:73
作者
Davies, MJ [1 ]
Hawkins, CL [1 ]
机构
[1] Heart Res Inst, Sydney, NSW 2050, Australia
基金
英国医学研究理事会; 英国惠康基金; 澳大利亚研究理事会;
关键词
spin trapping; electron paramagnetic resonance; protein oxidation; protein radicals; protein damage; free radicals;
D O I
10.1016/j.freeradbiomed.2003.12.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electron paramagnetic resonance (EPR) spin trapping was originally developed to aid the detection of low-molecular-mass radicals formed in chemical systems. It has subsequently found widespread use in biology and medicine for the direct detection of radical species formed during oxidative stress and via enzymatic reactions. Over the last 15 years this technique has also found increasing use in detecting and identifying radicals formed on biological macromolecules as a result of either radical reactions or enzymatic processes. Though the EPR signals that result from the trapping of large, slowly tumbling radicals are often broad and relatively poor in distinctive features, a number of techniques have been developed that allow a wealth of information to be obtained about the nature, site, and reactions of such radicals. This article summarizes recent developments in this area and reviews selected examples of radical formation on proteins. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:1072 / 1086
页数:15
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