Enzymatic Control of Chitosan Gelation for Delivery of Periodontal Ligament Cells

被引：12

作者：

Yan, Xiang-Zhen ^{[1
,2
]}

Nijhuis, Arnold W. G. ^{[1
]}

van den Beucken, Jeroen J. J. P. ^{[1
]}

Both, Sanne K. ^{[1
]}

Jansen, John A. ^{[1
]}

Leeuwenburgh, Sander C. G. ^{[1
]}

Yang, Fang ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Biomat, NL-6500 HB Nijmegen, Netherlands

[2] Shandong Univ, Dept Periodontol, Jinan 250012, Shandong, Peoples R China

来源：

MACROMOLECULAR BIOSCIENCE | 2014年 / 14卷 / 07期

关键词：

MESENCHYMAL STEM-CELLS; IN-VITRO EVALUATION; SPHEROID FORMATION; TISSUE; MICE; REGENERATION; HYDROGELS; BIOCOMPATIBILITY; DIFFERENTIATION; SYSTEM;

D O I：

10.1002/mabi.201400040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be controlled precisely by variation of the urea and urease concentrations. PDLCs remain viable inside these hydrogels for up to 30 days. Cells released from the hydrogel upon degradation and collected after 3, 15, and 30 days are able to form colonies and osteogenically differentiate. In conclusion, the enzymatic control over the gelation of chitosan hydrogels offers options for the delivery of PDLCs.

引用

收藏

页码：1004 / 1014

页数：11

相关论文

共 39 条

[1]

Effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice [J].

Burkatovskaya, Marina ;

Castano, Ana P. ;

Demidova-Rice, Tatiana N. ;

Tegos, George P. ;

Hamblin, Michael R. .

WOUND REPAIR AND REGENERATION, 2008, 16 (03) :425-431

[2]

Performance optimization of injectable chitosan hydrogel by combining physical and chemical triple crosslinking structure [J].

Chen, Chen ;

Wang, Lei ;

Deng, Liandong ;

Hu, Renjie ;

Dong, Anjie .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2013, 101 (03) :684-693

[3]

The influence of spheroid formation of human adipose-derived stem cells on chitosan films on sternness and differentiation capabilities [J].

Cheng, Nai-Chen ;

Wang, Shan ;

Young, Tai-Horng .

BIOMATERIALS, 2012, 33 (06) :1748-1758

[4]

Monolithic gelation of chitosan solutions via enzymatic hydrolysis of urea [J].

Chenite, A. ;

Gori, S. ;

Shive, M. ;

Desrosiers, E. ;

Buschmann, M. D. .

CARBOHYDRATE POLYMERS, 2006, 64 (03) :419-424

[5]

Novel injectable neutral solutions of chitosan form biodegradable gels in situ [J].

Chenite, A ;

Chaput, C ;

Wang, D ;

Combes, C ;

Buschmann, MD ;

Hoemann, CD ;

Leroux, JC ;

Atkinson, BL ;

Binette, F ;

Selmani, A .

BIOMATERIALS, 2000, 21 (21) :2155-2161

[6]

Physical gelation of chitosan in the presence of β-glycerophosphate:: The effect of temperature [J].

Cho, JY ;

Heuzey, MC ;

Bégin, A ;

Carreau, PJ .

BIOMACROMOLECULES, 2005, 6 (06) :3267-3275

[7]

Soft Substrates Promote Homogeneous Self-Renewal of Embryonic Stem Cells via Downregulating Cell-Matrix Tractions [J].

Chowdhury, Farhan ;

Li, Yanzhen ;

Poh, Yeh-Chuin ;

Yokohama-Tamaki, Tamaki ;

Wang, Ning ;

Tanaka, Tetsuya S. .

PLOS ONE, 2010, 5 (12)

[8]

Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice [J].

Chung, Mi Ja ;

Park, Jae Kweon ;

Park, Yong Il .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2012, 12 (02) :453-459

[9]

CIPOLLESCHI MG, 1993, BLOOD, V82, P2031

[10]

Development of bioactive and biodegradable chitosan-based injectable systems containing bioactive glass nanoparticles [J].

Couto, Daniela S. ;

Hong, Zhongkui ;

Mano, Joao F. .

ACTA BIOMATERIALIA, 2009, 5 (01) :115-123

← 1 2 3 4 →