Protein analysis of Babesia caballi merozoites by two-dimensional polyacrylamide gel electrophoresis and Western blotting

被引：3

作者：

Ikadai, H ^{[1
]}

Kabamoto, S ^{[1
]}

Xuan, X ^{[1
]}

Igarashi, I ^{[1
]}

Nagasawa, H ^{[1
]}

Fujisaki, K ^{[1
]}

Suzuki, N ^{[1
]}

Mikami, T ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Res Ctr Protozoan Mol Immunol, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2000年 / 62卷 / 03期

关键词：

Babesia caballi; 2D-PAGE; Western blotting;

D O I：

10.1292/jvms.62.323

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Babesia caballi merozoites were prepared by combining two improved methods of cultivation and purification of merozoites using Percoll-gradiation, and the protein compositions of merozoites were analyzed by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. The relative molecular masses of the major proteins and protein masses separated by electrophoresis were >94, 80-70, 50-45, 34-30, 30-28 and 18 kDa. By Western blotting, twelve proteins or protein groups were recognized by pooled sera from two horses experimentally infected with B. caballi. Among twelve proteins, five new proteins (54, 30-26, 24, and two 18 kDa) were identified, and the 48 kDa protein was revealed to consist of 2 components in the B. caballi merozoite. One protein (54 kDa) of B. caballi was also recognized by the pooled sera from two horses experimentally infected with B. equi.

引用

页码：323 / 327

页数：5

共 28 条

[1] PROTEIN CHARACTERIZATION OF BABESIA-EQUI PIROPLASMS ISOLATED FROM INFECTED HORSE ERYTHROCYTES [J].

ALI, S ;

SUGIMOTO, C ;

MATSUDA, M ;

SUGIURA, T ;

KANEMARU, T ;

ONUMA, M ;

KAMADA, M .

PARASITOLOGY RESEARCH, 1993, 79 (08) :639-643

[2] PHYLOGENY AND EVOLUTION OF THE PIROPLASMS [J].

ALLSOPP, MTEP ;

CAVALIERSMITH, T ;

DEWAAL, DT ;

ALLSOPP, BA .

PARASITOLOGY, 1994, 108 :147-152

[3] ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .21. 2-DIMENSIONAL ANALYSIS OF SERUM AND TISSUE PROTEINS - MULTIPLE ISOELECTRIC-FOCUSING [J].

ANDERSON, NG ;

ANDERSON, NL .

ANALYTICAL BIOCHEMISTRY, 1978, 85 (02) :331-340

[4] ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .22. 2-DIMENSIONAL ANALYSIS OF SERUM AND TISSUE PROTEINS - MULTIPLE GRADIENT-SLAB GEL-ELECTROPHORESIS [J].

ANDERSON, NL ;

ANDERSON, NG .

ANALYTICAL BIOCHEMISTRY, 1978, 85 (02) :341-354

[5] Improved in vitro cultivation of Babesia caballi [J].

Avarzed, A ;

Igarashi, I ;

Kanemaru, T ;

Hirumi, K ;

Omata, Y ;

Saito, A ;

Oyamada, T ;

Nagasawa, H ;

Toyoda, Y ;

Suzuki, N .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1997, 59 (06) :479-481

[6] Monoclonal antibody against Babesia equi:: Characterization and potential application of antigen for serodiagnosis [J].

Avarzed, A ;

Igarashi, I ;

De Waal, DT ;

Kawai, S ;

Oomori, Y ;

Inoue, N ;

Maki, Y ;

Omata, Y ;

Saito, A ;

Nagasawa, H ;

Toyoda, Y ;

Suzuki, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1835-1839

[7] ENRICHMENT OF BABESIA-CABALLI-INFECTED ERYTHROCYTES FROM MICROAEROPHILOUS STATIONARY-PHASE CULTURES USING PERCOLL GRADIENTS [J].

BHUSHAN, C ;

MULLER, I ;

FRIEDHOFF, KT .

PARASITOLOGY RESEARCH, 1991, 77 (02) :177-179

[8] POLYCLONAL ANTIBODY CHARACTERIZATION OF BABESIA-CABALLI ANTIGENS [J].

BOSE, R .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1994, 24 (04) :511-517

[9] IDENTIFICATION OF ANTIGENS DIAGNOSTIC FOR EUROPEAN ISOLATES OF BABESIA-EQUI BY 2-DIMENSIONAL ELECTROPHORESIS AND WESTERN BLOTTING [J].

BOSE, R ;

HENTRICH, B .

PARASITOLOGY RESEARCH, 1994, 80 (03) :182-185

[10] IDENTIFICATION OF DIAGNOSTIC ANTIGENS FOR SOUTH-AMERICAN BABESIA-CABALLI INFECTIONS [J].

BOSE, R ;

PEYMANN, B ;

BARBOSA, IP .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1994, 24 (02) :255-258

← 1 2 3 →