Improved in vitro cultivation of Babesia caballi

被引：56

作者：

Avarzed, A

Igarashi, I

Kanemaru, T

Hirumi, K

Omata, Y

Saito, A

Oyamada, T

Nagasawa, H

Toyoda, Y

Suzuki, N

机构：

[1] OBIHIRO UNIV AGR & VET MED,RES CTR PROTOZOAN MOL IMMUNOL,OBIHIRO,HOKKAIDO 080,JAPAN

[2] JAPAN RACING ASSOC,EQUINE RES CTR,EPIZOOT RES STN,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[3] OBIHIRO UNIV AGR & VET MED,DEPT VET PARASITOL,OBIHIRO,HOKKAIDO 080,JAPAN

[4] KITASATO UNIV,SCH VET MED & ANIM SCI,DEPT VET PARASITOL,TOWADA,AOMORI 034,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1997年 / 59卷 / 06期

关键词：

Babesia caballi; in vitro cultivation;

D O I：

10.1292/jvms.59.479

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O-2, 5% CO2 and 93% N-2. All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.

引用

页码：479 / 481

页数：3

共 14 条

[1] ENRICHMENT OF BABESIA-CABALLI-INFECTED ERYTHROCYTES FROM MICROAEROPHILOUS STATIONARY-PHASE CULTURES USING PERCOLL GRADIENTS [J].