The ESAT-6/WXG100 superfamily - and a new Gram-positive secretion system?

被引：280

作者：

Pallen, MJ ^{[1
]}

机构：

[1] Univ Birmingham, Med Sch, Div Immun & Infect, Birmingham B15 2TT, W Midlands, England

来源：

TRENDS IN MICROBIOLOGY | 2002年 / 10卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/S0966-842X(02)02345-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ESAT-6 is a small secreted protein of unknown function from Mycobacterium tuberculosis that is of fundamental importance in virulence and protective immunity. A PSI-BLAST search has identified distant homologues of ESAT-6 in more tractable bacteria, including Bacillus subtilis, Bacillus anthracis, Staphylococcus aureus and Clostridium acetobutylicum. The genes for ESAT-6-like proteins often cluster with genes encoding homologues of B. subtilis YukA. I speculate that the ESAT-6-like and YukA-like proteins form a novel Gram-positive secretion system potentially driven by the FtsK/SpoIIIE ATPase domains in the YukA-like proteins. The way is now open to investigate this hypothesis in organisms that are easier to manipulate than pathogenic mycobacteria.

引用

页码：209 / 212

页数：4

共 20 条

[11]

Neuwald AF, 1999, GENOME RES, V9, P27

[12] Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum [J].

Nölling, J ;

Breton, G ;

Omelchenko, MV ;

Makarova, KS ;

Zeng, QD ;

Gibson, R ;

Lee, HM ;

Dubois, J ;

Qiu, DY ;

Hitti, J ;

Wolf, YI ;

Tatusov, RL ;

Sabathe, F ;

Doucette-Stamm, L ;

Soucaille, P ;

Daly, MJ ;

Bennett, GN ;

Koonin, EV ;

Smith, DR .

JOURNAL OF BACTERIOLOGY, 2001, 183 (16) :4823-4838

[13] Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes [J].

Okinaka, RT ;

Cloud, K ;

Hampton, O ;

Hoffmaster, AR ;

Hill, KK ;

Keim, P ;

Koehler, TM ;

Lamke, G ;

Kumano, S ;

Mahillon, J ;

Manter, D ;

Martinez, Y ;

Ricke, D ;

Svensson, R ;

Jackson, PJ .

JOURNAL OF BACTERIOLOGY, 1999, 181 (20) :6509-6515

[14] Coiled-coil domains in proteins secreted by type III secretion systems [J].

Pallen, MJ ;

Dougan, G ;

Frankel, G .

MOLECULAR MICROBIOLOGY, 1997, 25 (02) :423-425

[15] Search for genes potentially involved in Mycobacterium tuberculosis virulence by mRNA differential display [J].

Rindi, L ;

Lari, N ;

Garzelli, CA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 258 (01) :94-101

[16] Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens [J].

Skjot, RLV ;

Oettinger, T ;

Rosenkrands, I ;

Ravn, P ;

Brock, I ;

Jacobsen, S ;

Andersen, P .

INFECTION AND IMMUNITY, 2000, 68 (01) :214-220

[17]

SORENSEN AL, 1995, INFECT IMMUN, V63, P1710

[18]

Tekaia F., 1999, Tubercle and Lung Disease, V79, P329, DOI 10.1054/tuld.1999.0220

[19]

VANPITTIUS NCG, 2001, GENOME BIOL, V2

[20] An esat6 knockout mutant of Mycobacterium bovis produced by homologous recombination will contribute to the development of a live tuberculosis vaccine [J].

Wards, BJ ;

de Lisle, GW ;

Collins, DM .

TUBERCLE AND LUNG DISEASE, 2000, 80 (4-5) :185-189

← 1 2 →