Novel cellulose/hydroxyapatite scaffolds for bone tissue regeneration: In vitro and in vivo study

被引:64
作者
Daugela, Povilas [1 ]
Pranskunas, Mindaugas [1 ]
Juodzbalys, Gintaras [1 ]
Liesiene, Jolanta [2 ]
Baniukaitiene, Odeta [2 ]
Afonso, Americo [3 ]
Gomes, Pedro Sousa [3 ,4 ]
机构
[1] Lithuanian Univ Hlth Sci, Dept Oral & Maxillofacial Surg, Eiveniu Str 2, LT-50009 Kaunas, Lithuania
[2] Kaunas Univ Technol, Dept Polymer Chem & Technol, Kaunas, Lithuania
[3] Univ Porto, Fac Dent Med, Porto, Portugal
[4] Univ Porto, REQUIMTE LAQV, Porto, Portugal
关键词
bone regeneration; bone tissue engineering; cellulose; hydroxyapatite; osteoblasts; porosity; MESENCHYMAL STEM-CELLS; NANO-HYDROXYAPATITE; BACTERIAL CELLULOSE; OSTEOGENIC DIFFERENTIATION; SERUM-PROTEINS; PROLIFERATION; MICRO; BIOCOMPATIBILITY; NANOCOMPOSITES; NANOPARTICLES;
D O I
10.1002/term.2651
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Cellulose scaffolds containing nano- or micro-hydroxyapatite (nHA or mu HA) were prepared by the regeneration of cellulose from its acetylated derivative and the mechanical immobilization of inorganic particles, followed by freeze-drying. Microtomographic (micro-computed tomography) evaluation revealed that both scaffolds presented a highly interconnected porous structure, with a mean pore diameter of 490 +/- 94 and 540 +/- 132 mu m for cellulose/nHA and cellulose/mu HA, respectively. In vitro and in vivo characterizations of the developed scaffolds were investigated. Commercially available bone allograft was used as a control material. For the in vitro characterization, osteoblastic cell cultures were used and characterized over time to evaluate cell adhesion, metabolic activity, and functional output (alkaline phosphatase activity and osteoblastic gene expression). The results revealed greater spreading cell distribution alongside an increased number of filopodia, higher MTT values, and significantly increased expression of osteoblastic genes (Runx-2, alkaline phosphatase, and BMP-2) for cellulose/nHA, compared with cellulose/HA and the control. The in vivo biocompatibility was evaluated in a rabbit calvarial defect model. The investigated scaffolds were implanted in circular rabbit calvaria defects. Four- and 12-week bone biopsies were investigated using micro-computed tomography and histological analysis. Although both cellulose/HA scaffolds outperformed the assayed control, a significantly higher amount of newly formed mineralized tissue was found within the defects loaded with cellulose/nHA. Within the limitations of this study, the developed cellulose/HA scaffolds showed promising results for bone regeneration applications. The biological response to the scaffold seems to be greatly dependent on the HA particles' characteristics, with cellulose scaffolds loaded with nHA eliciting an enhanced bone response.
引用
收藏
页码:1195 / 1208
页数:14
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