Oxygen sensitivity of red cell membrane transporters revisited

被引:20
作者
Drew, C
Ball, V
Robinson, H
Ellory, JC
Gibson, JS
机构
[1] Univ Cambridge, Dept Clin Vet Med, Cambridge CB3 0ES, England
[2] Univ Oxford, Physiol Lab, Oxford OX1 3PT, England
关键词
O-2; glut; KCC; NKCC; Na+/K+ pump; AE;
D O I
10.1016/j.bioelechem.2003.07.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, we provide an update on O-2-dependent membrane transport in red cells. O-2-sensitive membrane transport was compared in nucleated (chicken) and enucleated (human) red cells, to investigate effects on organic (glucose transporter [GLUT]) and inorganic (K+-Cl- cotransporter [KCC]Na+-K+-2Cl(-) cotransporter [NKCC]) transporters, to study the response of so-called "housekeeping" transporters (Na+/K+ pump and anion exchanger [AE]) and, finally, to compare O-2 sensitivity in normal human red cells with those from sickle cell patients. The Na+/K+ pump showed no change in activity between oxygenated and deoxygenated cells in any of the samples. KCC in normal human red cells had the greatest O-2 sensitivity, being stimulated some 20-fold on oxygenation. It was more modestly stimulated by O-2 in chicken red cells and HbS cells. By contrast, NKCC was stimulated by deoxygenation in all cases. GLUT showed little response to O-2 tension, other than a small stimulation in deoxygenated chicken red cells. Finally, AE I was stimulated by oxygenation in HbA cells, but this stimulation by O-2 was absent in HbS cells and pink ghosts prepared from HbA cells. The significance of these findings is discussed. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:153 / 158
页数:6
相关论文
共 33 条
[31]   Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter [J].
Speake, PF ;
Roberts, CA ;
Gibson, JS .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 273 (06) :C1811-C1818
[32]   POTASSIUM TRANSPORT IN DUCK RED CELLS [J].
TOSTESON, DC ;
ROBERTSON, JS .
JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1956, 47 (01) :147-166
[33]  
WOOD RE, 1969, J BIOL CHEM, V244, P1451