Retrotransposon-based S-SAP as a platform for the analysis of genetic variation and linkage in globe artichoke

被引：14

作者：

Acquadro, Alberto ^{[1
]}

Portis, Ezio ^{[1
]}

Moglia, Andrea ^{[1
]}

Magurno, Franco ^{[1
]}

Lanteri, Sergio ^{[1
]}

机构：

[1] Univ Turin, I-00195 Grugliasco, Turin, Italy

来源：

GENOME | 2006年 / 49卷 / 09期

关键词：

Cynara cardunculus; retrotransposons; S-SAP; genetic variation; linkage analysis;

D O I：

10.1139/G06-074

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A high copy number of retrotransposon sequences are present and widely dispersed in plant genomes. Their activity generates a considerable degree of sequence polymorphism. Here, we report the cloning of CYRE-5, a long-terminal repeat carrying retrotransposon-like sequence in Cynara cordunculus L., and its exploitation to develop a DNA fingerprinting assay across 22 accessions, including both cultivated (globe artichoke and cultivated cardoon) and wild (wild cardoon) types. The effectiveness of the sequence-specific amplified polymorphism (S-SAP) platform is compared with that of amplified fragment length polymorphism (AFLP). A genetic linkage analysis, based on a hybrid population between 2 globe artichoke varietal types, resulted in the inclusion of 29 S-SAP loci in the core genetic map, confirming their dispersed distribution across the globe artichoke genome.

引用

页码：1149 / 1159

页数：11

共 72 条

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