Bioimaging TOF-SIMS: localization of cholesterol in rat kidney sections

被引：92

作者：

Nygren, H

Malmberg, P

Kriegeskotte, C

Arlinghaus, HF

机构：

[1] Univ Gothenburg, Dept Anat & Cell Biol, SE-40530 Gothenburg, Sweden

[2] Univ Munster, Inst Phys, D-4400 Munster, Germany

来源：

FEBS LETTERS | 2004年 / 566卷 / 1-3期

关键词：

cholesterol; localization; kidney; TOF-SIMS; silver matrix;

D O I：

10.1016/j.febslet.2004.04.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we show the localization of a whole organic molecule in biological tissue using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Rat kidneys were sectioned by cryoultramicrotomy and dried at room temperature. The samples were covered with a thin silver layer and analyzed in an imaging TOF-SIMS instrument equipped with a Ga+-source. The cholesterol signal showed a high concentration in the nuclear areas of the epithelial cells and a lower concentration over areas representing the basal lamina of renal tubules. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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页码：291 / 293

页数：3

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