Detrimental effect of the combination of R164S with G238S in TEM-1 β-lactamase on the extended-spectrum activity conferred by each single mutation

被引：20

作者：

Giakkoupi, P

Tzelepi, E

Tassios, PT

Legakis, NJ

Tzouvelekis, LS

机构：

[1] Univ Athens, Sch Med, Dept Microbiol, Lab Antimicrobial Agents, GR-11527 Athens, Greece

[2] Hellenic Pasteur Inst, Dept Bacteriol, Athens, Greece

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2000年 / 45卷 / 01期

关键词：

D O I：

10.1093/jac/45.1.101

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The non-naturally occurring TEM-1 beta-lactamase mutant R164S:G238S, as well as the R164S (TEM-12) and G238S (TEM-19) beta-lactamases, were constructed and expressed in Escherichia coli under isogenic conditions. Comparison of susceptibilities to beta-lactam antibiotics and substrate profiles showed that the combination of R164S with G238S drastically reduced the extended-spectrum activity of the respective single mutants.

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页码：101 / 104

页数：4

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