Biochemical preparation of L-ribose and L-arabinose from ribitol: A new approach

被引：48

作者：

Ahmed, Z ^{[1
]}

Shimonishi, T ^{[1
]}

Bhuiyan, SH ^{[1
]}

Utamura, M ^{[1
]}

Takada, G ^{[1
]}

Izumori, K ^{[1
]}

机构：

[1] Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Miki, Kagawa 7610795, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 1999年 / 88卷 / 04期

关键词：

ribitol; L-ribose; L-arabinose; L-ribose isomerase; L-arabinose isomerase;

D O I：

10.1016/S1389-1723(99)80225-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

L-Ribose and L-arabinose were prepared biochemically from ribitol via a two-step reaction, by which the complete oxidation of ribitol to L-ribulose (similar to 98%) was achieved by the reaction of washed cells of Acetobacter aceti IFO 3281. The produced L-ribulose was then used as a substrate for the production of L-ribose and L-arabinose. The isomerization of L-ribulose to L-ribose and L-arabinose was carried out using L-ribose isomerase (L-RI) of Acinetobacter sp, strain DL-28 and L-arabinose isomerase (L-AI) of Mycobacterium smegmatis, respectively. At equilibrium, the ratio of L-ribose : L-ribulose was 70 : 30 and that of L-arabinose : L-ribulose was 90 : 10. After a simple purification treatment, both pentoses could be crystallized without the use of column chromatography. The crystals were confirmed as L-ribose and L-arabinose by High-performance liquid chromatography (HPLC), Infrared (IR), Nuclear magnetic resonance (NMR) and optical rotation measurements.

引用

页码：444 / 448

页数：5

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