Isolation and preliminary characterization of a Zymomonas mobilis mutant with an altered preference for xylose and glucose utilization

被引：12

作者：

Supple, SG ^{[1
]}

Joachimsthal, EL ^{[1
]}

Dunn, NW ^{[1
]}

Rogers, PL ^{[1
]}

机构：

[1] Univ New S Wales, Dept Biotechnol, Kensington, NSW 2052, Australia

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 02期

关键词：

ethanol; glucose; pentoses; xylose transport; Zymomonas mobilis;

D O I：

10.1023/A:1005613912777

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The narrow substrate range of Zymomonas mobilis CP4 has been extended previously to include metabolism of the pentose sugar, xylose, by Zhang et al. (Science 267: 240-243). The strain CP4(pZB5) co-ferments both glucose and xylose in mixed sugar fermentations, however glucose is utilized preferentially. The present work reports the isolation of a new mutant from CP4(pZB5) which displays an altered carbon substrate preference. The mutant, CP4(pZB5) M1-2, metabolizes xylose more rapidly than glucose in mixed glucose/xylose media. Sequence data analysis revealed mutations in both the glucose facilitator (glf) and glucokinase (glk) genes.

引用

页码：157 / 164

页数：8