Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform

被引：28

作者：

Kim, Yun-Gon ^{[1
,2
]}

Jeong, Hee-Jin ^{[3
]}

Jang, Kyoung-Soon ^{[3
]}

Yang, Yung-Hun ^{[3
]}

Song, Yong-Sang ^{[4
]}

Chung, Junho ^{[5
]}

Kim, Byung-Gee ^{[1
,2
,3
]}

机构：

[1] Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151742, South Korea

[2] Seoul Natl Univ, Inst Bioengn, Seoul 151742, South Korea

[3] Seoul Natl Univ, Coll Engn, Sch Chem & Biol Engn, Seoul 151742, South Korea

[4] Seoul Natl Univ, Coll Med, Dept Obstet & Gynecol, Seoul 110744, South Korea

[5] Seoul Natl Univ, Coll Med, Dept Biochem & Mol Biol, Seoul 110744, South Korea

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 391卷 / 02期

关键词：

OLIGOSACCHARIDES; GLYCOSYLATION; PROTEINS;

D O I：

10.1016/j.ab.2009.05.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures, The released N-glycans front polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan Preparation technology is applied to ovarian cancer diagnosis using 5 mu l of patient Serum. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：151 / 153

页数：3

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