Metabolic activity of bacterioplankton communities assessed by flow cytometry and single carbon substrate utilization

被引:69
作者
Jellett, JF [1 ]
Li, WKW [1 ]
Dickie, PM [1 ]
Boraie, A [1 ]
Kepkay, PE [1 ]
机构
[1] FISHERIES & OCEANS CANADA, BEDFORD INST OCEANOG, DEPT FISHERIES & OCEANS, BIOL OCEANOG SECT, DARTMOUTH, NS B2Y 4A2, CANADA
关键词
flow cytometry; nucleic acid content; TO-PRO(TM); Biolog; tritiated leucine; tritiated thymidine; cell cycle;
D O I
10.3354/meps136213
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
During the summer of 1994, bacterioplankton abundance and metabolism were examined in seawater from Bedford Basin, Nova Scotia, Canada. Two new methods were applied to independently assess metabolic activity: (1) flow cytometric analysis of bimodal nucleic acid distributions in bacterioplankton stained with a novel fluorescent dye, and (2) utilization of single carbon substrates in Biolog GN Microplates(TM). Both sets of results were compared to bacterial production estimates obtained using a standard technique for H-3-thymidine and H-3-leucine incorporation. Flow cytometry quantified the relative abundance of the bacterioplankton cells with a high apparent nucleic acid content, which was expressed as an active cell index (ACI) of dividing and/or actively metabolizing cells. The ACI was positively correlated with sole carbon source utilization at 1, 5 and 10 m (p < 0.05). Variations in ACI and sole carbon source utilization followed trends similar to, but could not be directly correlated with, bacterial production throughout the summer period. The new methods provided information that could not be obtained using the standard techniques for bacterial production. Instead, they yielded new and complementary information on the metabolic state of dividing and/or metabolizing cells and insight into the regulation of bacterial production.
引用
收藏
页码:213 / 225
页数:13
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