TRAIL-induced apoptosis proceeding from caspase-3-dependent and -independent pathways in distinct HeLa cells

被引：33

作者：

Lin, Juqiang ^{[1
]}

Zhang, Zhihong ^{[1
]}

Zeng, Shaoqun ^{[1
]}

Zhou, Shixia ^{[1
]}

Liu, Bi-Feng ^{[1
]}

Liu, Qian ^{[1
]}

Yang, Jie ^{[1
]}

Luo, Qingming ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Key Lab Biomed Photon, Minist Educ, Wuhan 430074, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 346卷 / 04期

基金：

中国国家自然科学基金;

关键词：

caspase; fluorescence resonance energy transfer (FRET); tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL); capillary electrophoresis (CE);

D O I：

10.1016/j.bbrc.2006.05.209

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The apoptotic pathway in higher eukaryotes remains controversial with respect to the necessity of activation of caspase-3 in TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-treated cells. In this study, a fluorescence resonance energy transfer (FRET) probe was developed to image the activation of caspase-3 and the related apoptotic pathway in TRAIL-treated cells in real time. Both kinds of apoptotic pathways were observed simultaneously in the same experiment proceeding from activation and non-activation of caspase-3. The total apoptotic rate was 56.08%, the apoptotic rates for activation and non-activation of caspase-3 pathways were 21.5% and 34.58%, respectively, which were examined later for Hoechst 33258 staining and morphological characteristics. The apoptotic rate due to the activation of caspase-3 pathways in TRAIL-treated cells has been independently measured to be around 25.11% by capillary electrophoresis (CE) analysis, which confirmed the apoptotic rate due to activation of caspase-3 pathways as found by FRET analysis. This result also suggests that rest apoptosis is preceded by caspase-3-independent pathways, as CE has the ability to quantitatively detect caspase-dependent apoptosis. The observation of the coexistence of caspase-3-dependent and caspase- 3 -independent apoptotic pathways in the TRAIL-treated cells was unusual in comparison with the previous reports. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1136 / 1141

页数：6

共 23 条

[1] DsRed as a potential FRET partner with CFP and GFP [J].