NMR in the SPINE structural proteomics project

被引：13

作者：

Ab, E.

Atkinson, A. R.

Banci, L.

Bertini, I.

Ciofi-Baffoni, S.

Brunner, K.

Diercks, T.

Doetsch, V.

Engelke, F.

Folkers, G. E.

Griesinger, C.

Gronwald, W.

Guenther, U.

Habeck, M.

de Jong, R. N.

Kalbitzer, H. R.

Kieffer, B.

Leeflang, B. R.

Loss, S.

Luchinat, C.

Marquardsen, T.

Moskau, D.

Neidig, K. -P.

Nilges, M.

Piccioli, M.

Pierattelli, R.

Rieping, W.

Schippmann, T.

Schwalbe, H.

Trave, G.

Trenner, J.

Woehnert, J.

Zweckstetter, M.

Kaptein, R.

机构：

[1] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 CH Utrecht, Netherlands

[2] Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France

[3] Univ Florence, CIRMMP, CERM, I-50019 Sesto Fiorentino, Italy

[4] Univ Regensburg, Dept Biophys & Phys Biochem, D-8400 Regensburg, Germany

[5] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, D-6000 Frankfurt, Germany

[6] Bruker Biospin GmbH, Rheinstetten, Germany

[7] Max Planck Inst Biophys Chem, Gottingen, Germany

[8] Inst Pasteur, F-75724 Paris, France

[9] Bruker Biospin AG, Fallanden, Switzerland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷

关键词：

D O I：

10.1107/S0907444906032070

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe ( SPINE) consortium. Focusing on the development of high- throughput ( HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless C-13-direct detection methods and inferential structure determinations ( ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.

引用

页码：1150 / 1161

页数：12

共 82 条

[81] Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings [J].