Closing gaps in the human genome using sequencing by synthesis

被引：18

作者：

Garber, Manuel ^{[1
]}

Zody, Michael C. ^{[1
,2
]}

Arachchi, Harindra M. ^{[1
]}

Berlin, Aaron ^{[1
]}

Gnerre, Sante ^{[1
]}

Green, Lisa M. ^{[1
]}

Lennon, Niall ^{[1
]}

Nusbaum, Chad ^{[1
]}

机构：

[1] Broad Inst MIT & Harvard, Genome Sequencing & Anal Program, Cambridge, MA 02142 USA

[2] Uppsala Univ, Dept Med Biochem & Microbiol, SE-75124 Uppsala, Sweden

来源：

GENOME BIOLOGY | 2009年 / 10卷 / 06期

关键词：

HIGH-DENSITY;

D O I：

10.1186/gb-2009-10-6-r60

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15.

引用

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