共 41 条
RhoGDI2 Expression Is Associated with Tumor Growth and Malignant Progression of Gastric Cancer
被引:78
作者:
Cho, Hee Jun
[1
]
Baek, Kyoung Eun
[1
]
Park, Sun-Mi
[1
]
Kim, In-Kyu
[1
]
Choi, Yeong-Lim
[1
]
Cho, Hye-Jung
[1
]
Nam, In-Koo
[1
]
Hwang, Eun Mi
Park, Jae-Yong
Han, Jae Yoon
[2
,3
]
Kang, Sang Soo
[2
,3
]
Kim, Dong Chul
Lee, Won Sup
Lee, Mi-Ni
[4
]
Oh, Goo Taeg
[4
]
Kim, Jae Won
[1
]
Lee, Chang Won
[1
]
Yoo, Jiyun
[1
]
机构:
[1] Gyeongsang Natl Univ, Dept Microbiol, Life Sci Res Inst, Coll Nat Sci, Jinju 660701, South Korea
[2] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Jinju 660701, South Korea
[3] Gyeongsang Natl Univ, Sch Med, Med Res Ctr Neural Dysfunct, Jinju 660701, South Korea
[4] Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul, South Korea
关键词:
GDP-DISSOCIATION INHIBITOR;
CARCINOMA CELL-LINES;
GTP-BINDING PROTEINS;
RHO-GTPASES;
LY-GDI;
METASTASIS;
INVASION;
CYCLOOXYGENASE-2;
GENE;
IDENTIFICATION;
D O I:
10.1158/1078-0432.CCR-08-2192
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
Purpose: Rho GDP dissociation inhibitor 2 (RhoGDI2) has been identified as a regulator of Rho family GTPase. However, there is currently no direct evidence suggesting whether RhoGDI2 activates or inhibits Rho family GTPase in vivo (and which type), and the role of RhoGDI2 in tumor remains controversial. Here, we assessed the effects of RhoGDI2 expression on gastric tumor growth and metastasis progression. Experimental Design: Proteomic analysis was done to investigate the tumor-specific protein expression in gastric cancer and RhoGDI2 was selected for further study. Immunohistochemistry was used to detect RhoGDI2 expression in clinical samples of primary gastric tumor tissues which have different pathologic stages. Gain-of-function and loss-of-function approaches were done to examine the malignant phenotypes of the Rho GDI2-expressing or RhoGDI2-depleting cells. Results: RhoGDI2 expression was correlated positively with tumor progression and metastasis potential in human gastric tumor tissues, as well as cell lines. The forced expression of RhoGDI2 caused a significant increase in gastric cancer cell invasion in vitro, and tumor growth, angiogenesis, and metastasis in vivo, whereas RhoGDI2 depletion evidenced opposite effects. Conclusion: Our findings indicate that RhoGDI2 is involved in gastric tumor growth and metastasis, and that RhoGDI2 may be a useful marker for tumor progression of human gastric cancer.
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页码:2612 / 2619
页数:8
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