Selectivity-enhancement in enantioselective hydrolysis of sec-alkyl sulfates by an alkylsulfatase from Rhodococcus ruber DSM 44541

被引：29

作者：

Pogorevc, M

Strauss, UT

Riermeier, T

Faber, K

机构：

[1] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

[2] Degussa AG, Projekthaus Katalyse, D-65926 Frankfurt, Germany

来源：

TETRAHEDRON-ASYMMETRY | 2002年 / 13卷 / 13期

关键词：

D O I：

10.1016/S0957-4166(02)00363-4

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The Enantioselectivity of the biohydrolysis of sec-alkyl sulfate esters using a bacterial alkylsulfatase from Rhodococcus ruber DSM 44541 was dramatically enhanced in presence of additives ('enhancers') such as carbohydrates, polyethylene glycol, detergents, metal ions and through enzyme immobilization. In presence of iron, the E value for the kinetic resolution of (+/-)-3- and (+/-)-4-octyl sulfate was improved from E= 3.9 to greater than or equal to 200 and E= 1.1 to 10, respectively. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1443 / 1447

页数：5

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