SELECTIVITY-ENHANCEMENT OF HYDROLASE REACTIONS

被引：68

作者：

FABER, K

OTTOLINA, G

RIVA, S

机构：

[1] GRAZ UNIV TECHNOL, INST ORGAN CHEM, A-8010 GRAZ, AUSTRIA

[2] CNR, IST CHIM ORMONI, I-20131 MILAN, ITALY

来源：

BIOCATALYSIS | 1993年 / 8卷 / 02期

关键词：

ESTERASE; PROTEASE; LIPASE; SELECTIVITY;

D O I：

10.3109/10242429308998200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review pinpoints the strategies which can be employed to improve the enantio- and diasteroselectivity of hydrolytic enzymes, i.e. esterases, proteases, and lipases. The influence of variations of reactants, - enzyme and substrate - and conditions - kinetics, medium, temperature, pH - on the chiral recognition process of the enzyme is discussed with examples from the recent literature.

引用

页码：91 / 132

页数：42

共 135 条

[1]

ADACHI K, 1986, CHIMIA, V40, P311

[2]

Ammon R, 1931, BIOCHEM Z, V234, P54

[3] TEMPERATURE AND DMSO INCREASE THE ENANTIOSELECTIVITY OF HYDROLYSIS OF METHYL ALKYL DIMETHYLMALONATES CATALYZED BY PIG-LIVER ESTERASE [J].