Identification of Haemophilus influenzae and Haemophilus haemolyticus by matrix-assisted laser desorption ionization-time of flight mass spectrometry

被引：26

作者：

Bruin, J. P. ^{[1
]}

Kostrzewa, M. ^{[2
]}

van der Ende, A. ^{[3
]}

Badoux, P. ^{[1
]}

Jansen, R. ^{[1
]}

Boers, S. A. ^{[1
]}

Diederen, B. M. W. ^{[1
,4
]}

机构：

[1] Reg Publ Hlth Lab, Haarlem, Netherlands

[2] Bruker Daltonik GmbH, Bremen, Germany

[3] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands

[4] Univ Amsterdam, Acad Med Ctr, Netherlands Reference Lab Bacterial Meningitis, NL-1105 AZ Amsterdam, Netherlands

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2014年 / 33卷 / 02期

关键词：

COLONIZATION; DISEASE; PCR;

D O I：

10.1007/s10096-013-1958-x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Generally accepted laboratory methods that have been used for decades do not reliably distinguish between H. influenzae and H. haemolyticus isolates. H. haemolyticus strains are often incorrectly identified as nontypeable Haemophilus influenzae (NTHi). To distinguish H. influenzae from H. haemolyticus we have created a new database on the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) bio-typer 2 and compared the results with routine determination of Haemophilus (growth requirement for X and V factor), and multilocus sequence typing (MLST). In total we have tested 277 isolates, 244 H. influenzae and 33 H. haemolyticus. Using MLST as the gold standard, the agreement of MALDI-TOF MS was 99.6 %. MALDI-TOF MS allows reliable and rapid discrimination between H. influenzae and H. haemolyticus.

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收藏

页码：279 / 284

页数：6

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