SK-ER3 neuroblastoma cells as a model for the study of estrogen influence on neural cells

被引：36

作者：

Agrati, P ^{[1
]}

Garnier, M ^{[1
]}

Patrone, C ^{[1
]}

Pollio, G ^{[1
]}

Santagati, S ^{[1
]}

Vegeto, E ^{[1
]}

Maggi, A ^{[1
]}

机构：

[1] UNIV MILAN, INST PHARMACOL SCI, CTR MPL, I-20133 MILAN, ITALY

来源：

BRAIN RESEARCH BULLETIN | 1997年 / 44卷 / 04期

关键词：

neuroblastoma; stable transfection; estrogen receptor; differentiation; brain;

D O I：

10.1016/S0361-9230(97)00237-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The neuroblastoma SK-ER3 cell line obtained by stable transfection of the human SK-N-BE cell line is proposed as a model for the study of estrogen receptor activity in cells of neural origin, In the SK-ER3 cell line the estrogen receptor, once activated, initiates a differentiation program leading to growth arrest, morphological changes, and acquisition of the dopaminergic phenotype, In the absence of estrogens, this program can be triggered by IGF-I, which can activate the unliganded estrogen receptor via the ras-pathway, It is proposed that this model system might recapitulate the events occurring in vivo during the differentiation of the nervous system and that IGF-I may play an important role in the activation of estrogen receptor at the very early stage of brain development affecting the differentiation of a number of hypothalamic and extrahypothalamic brain regions. (C) 1997 Elsevier Science Inc.

引用

页码：519 / 523

页数：5

共 19 条

[11] DEVELOPMENT OF ESTROGEN-RECEPTOR SYSTEMS IN THE RAT-BRAIN - PERINATAL-DEVELOPMENT [J].

MACLUSKY, NJ ;

LIEBERBURG, I ;

MCEWEN, BS .

BRAIN RESEARCH, 1979, 178 (01) :129-142

[12]

O'Malley B W, 1969, Recent Prog Horm Res, V25, P105

[13] TRANSIENT ELEVATION OF ESTROGEN-RECEPTORS IN THE NEONATAL RAT HIPPOCAMPUS [J].

OKEEFE, JA ;

HANDA, RJ .

DEVELOPMENTAL BRAIN RESEARCH, 1990, 57 (01) :119-127

[14] Cross-coupling between insulin and estrogen receptor in human neuroblastoma cells [J].

Patrone, C ;

Ma, ZQ ;

Pollio, G ;

Agrati, P ;

Parker, MG ;

Maggi, A .

MOLECULAR ENDOCRINOLOGY, 1996, 10 (05) :499-507

[15] SEXUAL DIMORPHISM IN NEUROPIL OF PREOPTIC AREA OF RAT AND ITS DEPENDENCE ON NEONATAL ANDROGEN [J].

RAISMAN, G ;

FIELD, PM .

BRAIN RESEARCH, 1973, 54 (MAY17) :1-29

[16] THE EFFECTS OF ESTRADIOL ON THE GROWTH-PATTERNS OF ESTROGEN RECEPTOR-POSITIVE HYPOTHALAMIC CELL-LINES [J].

RASMUSSEN, JE ;

TORRESALEMAN, I ;

MACLUSKY, NJ ;

NAFTOLIN, F ;

ROBBINS, RJ .

ENDOCRINOLOGY, 1990, 126 (01) :235-240

[17]

SOHRABJI F, 1994, J NEUROSCI, V14, P459

[18] CULTURE OF RAT-BRAIN PREOPTIC AREA NEURONS - EFFECTS OF SEX STEROIDS [J].

TAKAGI, K ;

KAWASHIMA, S .

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1993, 11 (01) :63-70

[19] Molecular analysis of a steroid-induced regulatory hierarchy: The Drosophila E74A protein directly regulates L71-6 transcription [J].

Urness, LD ;

Thummel, CS .

EMBO JOURNAL, 1995, 14 (24) :6239-6246

← 1 2 →