Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts

被引:180
作者
Lamb, RF
Ozanne, BW
Roy, C
McGarry, L
Stipp, C
Mangeat, P
Jay, DG
机构
[1] UNIV MONTPELLIER 2, CNRS, UMR 5539, F-34095 MONTPELLIER 5, FRANCE
[2] HARVARD UNIV, DEPT MOL & CELLULAR BIOL, CAMBRIDGE, MA 02138 USA
基金
英国医学研究理事会;
关键词
D O I
10.1016/S0960-9822(06)00295-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Changes in cell shape and motility are important manifestations of oncogenic transformation, hut the mechanisms underlying these changes and key effector molecules in the cytoskeleton remain unknown. The Fos oncogene induces expression of ezrin, the founder member of the ezrin/radixin/moesin (ERM) protein family, but not expression of the related ERM proteins, suggesting that ezrin has a distinct role in cell transformation. ERM proteins have been suggested to link the plasma membrane to the actin-based cytoskeleton and are substrates and anchoring sites for a variety of protein kinases. Here, we examined the role of ezrin in cellular transformation. Results: Fos-mediated transformation of Rat-1 fibroblasts resulted in an increased expression and hyperphosphorylation of ezrin, and a concomitant increased association of ezrin with the cortical cytoskeleton. We lagged ezrin with green fluorescent protein and examined its distribution in normal and Fos-transformed fibroblasts: ezrin was concentrated at the leading edge of extending pseudopodia of Fos-transformed Rat-1 cells, and was mainly cytosolic in normal Rat-1 cells. Functional ablation of ezrin by micro-CALI (chromophore-assisted laser inactivation) blocked plasma-membrane ruffling and motility of Fos-transformed fibroblasts. Ablation of ezrin in normal Rat-1 cells caused a marked collapse of the leading edge of the cell, Conclusion: Ezrin plays an important role in pseudopodial extension in Fos-transformed Rat-1 fibroblasts, and maintains cell shape. in normal Rat-1 cells, The increased expression, hyperphosphorylation and subcellular redistribution of ezrin upon fibroblast transformation coupled with ifs roles in cell shape and motility suggest a critical role for ezrin in oncogenic transformation.
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收藏
页码:682 / 688
页数:7
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