Improvement of CHO cell culture medium formulation:: Simultaneous substitution of glucose and glutamine

被引:155
作者
Altamirano, C [1 ]
Paredes, C [1 ]
Cairó, JJ [1 ]
Gòdia, F [1 ]
机构
[1] Univ Autonoma Barcelona, Escola Tecn Super Engn, Dept Engn Quim, E-08193 Barcelona, Spain
关键词
D O I
10.1021/bp990124j
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The formulation of the culture medium for a Chinese hamster ovary (CHO) cell line has been investigated in terms of the simultaneous replacement of glucose and glutamine, the most commonly employed carbon and nitrogen sources, pursuing the objective of achieving a more efficient use of these compounds, simultaneously avoiding the accumulation of lactate and ammonium in the medium. The key factor in this process is the selection of compounds that are slowly metabolized. Among the different compounds studied, galactose and glutamate provide the best results, allowing support of cell growth with an optimal balance between nutrient uptake and cell requirements and the generation of minimal quantities of lactate and ammonium. The attained results also highlight the capacity of the cells to redistribute their metabolism as a response to the changes in medium composition.
引用
收藏
页码:69 / 75
页数:7
相关论文
共 30 条
[1]   IN PURSUIT OF THE OPTIMAL FED-BATCH PROCESS FOR MONOCLONAL-ANTIBODY PRODUCTION [J].
BIBILA, TA ;
ROBINSON, DK .
BIOTECHNOLOGY PROGRESS, 1995, 11 (01) :1-13
[2]   THE EFFECTS OF GLUTAMINE UTILIZATION AND AMMONIA PRODUCTION ON THE GROWTH OF BHK CELLS IN MICROCARRIER CULTURES [J].
BUTLER, M ;
SPIER, RE .
JOURNAL OF BIOTECHNOLOGY, 1984, 1 (3-4) :187-196
[3]   DETERMINATION OF AMMONIUM AND L-GLUTAMINE IN HYBRIDOMA CELL-CULTURES BY SEQUENTIAL FLOW-INJECTION ANALYSIS [J].
CAMPMAJO, C ;
CAIRO, JJ ;
SANFELIU, A ;
MARTINEZ, E ;
ALEGRET, S ;
GODIA, F .
CYTOTECHNOLOGY, 1994, 14 (03) :177-182
[4]  
CASTRO PML, 1992, APPL MICROBIOL BIOT, V38, P84
[5]   INFLUENCE OF AMMONIUM ON GROWTH, METABOLISM, AND PRODUCTIVITY OF A CONTINUOUS SUSPENSION CHINESE-HAMSTER OVARY CELL-CULTURE [J].
HANSEN, HA ;
EMBORG, C .
BIOTECHNOLOGY PROGRESS, 1994, 10 (01) :121-124
[6]   DEVELOPMENT OF A SERUM-FREE CULTURE-MEDIUM FOR THE LARGE-SCALE PRODUCTION OF RECOMBINANT PROTEIN FROM A CHINESE-HAMSTER OVARY CELL-LINE [J].
KEEN, MJ ;
RAPSON, NT .
CYTOTECHNOLOGY, 1995, 17 (03) :153-163
[7]  
KOVAR J, 1986, METHOD ENZYMOL, V121, P277
[8]   GROWTH-BEHAVIOR OF CHINESE HAMSTER OVARY CELLS IN A COMPACT LOOP BIOREACTOR .2. EFFECTS OF MEDIUM COMPONENTS AND WASTE PRODUCTS [J].
KURANO, N ;
LEIST, C ;
MESSI, F ;
KURANO, S ;
FIECHTER, A .
JOURNAL OF BIOTECHNOLOGY, 1990, 15 (1-2) :113-128
[9]   Development of a serum-free medium using computer-assisted factorial design and analysis [J].
Lao, MS ;
Schalla, C .
CYTOTECHNOLOGY, 1996, 22 (1-3) :25-31
[10]   CATABOLIC CONTROL OF HYBRIDOMA CELLS BY GLUCOSE AND GLUTAMINE LIMITED FED-BATCH CULTURES [J].
LJUNGGREN, J ;
HAGGSTROM, L .
BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (07) :808-818