Direct monitoring of biochemical processes using micro-structured heat power detectors

被引：13

作者：

Lerchner, J ^{[1
]}

Wolf, A ^{[1
]}

Hüttl, R ^{[1
]}

Wolf, G ^{[1
]}

机构：

[1] TU Bergakad Freiberg, Inst Phys Chem, D-09596 Freiberg, Germany

来源：

CHEMICAL ENGINEERING JOURNAL | 2004年 / 101卷 / 1-3期

关键词：

calorimetry; flow-through chip calorimeter; enzymes; micro-kelvin thermostat; degree of mixing;

D O I：

10.1016/j.cej.2003.11.006

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

An improved flow-through chip calorimeter useful for liquid samples is presented. The heat power detection limit could be decreased to a level of 180 nW. Main features of the new micro-sized calorimeter are a differential chip arrangement, a small sized thermostat with a precision in the micro-kelvin range and the application of micro-pumps for liquid flow control. The improved heat power resolution enables the monitoring of biochemical processes under relevant conditions. As applications have shown a pulsed fluid injection can increase the degree of mixing. For the determination of the degree of mixing a new method was developed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：187 / 194

页数：8

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