Fish testicular 11 beta-hydroxylase: cDNA cloning and mRNA expression during spermatogenesis

被引：44

作者：

Jiang, JQ

Kobayashi, T

Ge, W

Kobayashi, H

Tanaka, M

Okamoto, M

Nonaka, Y

Nagahama, Y

机构：

[1] NATL INST BASIC BIOL,REPROD BIOL LAB,OKAZAKI,AICHI 444,JAPAN

[2] OSAKA UNIV,SCH MED,DEPT MOL PHYSIOL CHEM,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1996年 / 397卷 / 2-3期

关键词：

cytochrome P450(11 beta); cDNA cloning; spermatogenesis; Japanese eel;

D O I：

10.1016/S0014-5793(96)01187-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated and characterized a cDNA encoding testicular 11 beta-hydroxylase, cytochrome P450(11 beta) from the Japanese eel (Anguilla japonica) testis, The cDNA contains an open reading frame that encodes a protein of 511 amino acids, The predicted amino acid sequence shares 38-48% homology with those of adrenal P450(11 beta) from mammals and frog. Transient expression in COS 1 cells confirmed that the protein encoded by this cDNA had P450(11 beta) activity, Northern blotting revealed a single 1.8 kb long transcript of P450(11 beta), This transcript was not found in immature eel testes prior to an injection with human chorionic gonadotropin (hCG), but it was present in eel testes after hCG injection.

引用

页码：250 / 252

页数：3

共 16 条

[1] CLONING AND EXPRESSION ANALYSIS OF A CYTOCHROME P-450(11-BETA) CDNA IN SHEEP [J].