Assessment of mechanical properties of adherent living cells by bead micromanipulation:: Comparison of magnetic twisting cytometry vs optical tweezers

被引：139

作者：

Laurent, VM

Hénon, S

Planus, E

Fodil, R

Balland, M

Isabey, D

Gallet, F

机构：

[1] Univ Paris 06, CNRS, ESA 7057, Lab Biorheol & Hydrodynam Physicochim, F-75251 Paris 5, France

[2] Univ Paris 07, CNRS, ESA 7057, Lab Biorheol & Hydrodynam Physicochim, F-75251 Paris, France

[3] Univ Paris 12, INSERM, Unite Physiopathol & Therapeut Resp 492, Fac Med, F-94010 Creteil, France

[4] Univ Paris 12, INSERM, Unite Physiopathol & Therapeut Resp 492, Fac Sci & Technol, F-94010 Creteil, France

[5] Univ Paris 06, Federat Rech Matiere & Syst Complexes, F-75251 Paris 5, France

[6] Univ Paris 07, Federat Rech Matiere & Syst Complexes, F-75251 Paris 5, France

来源：

JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME | 2002年 / 124卷 / 04期

关键词：

D O I：

10.1115/1.1485285

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We compare the measurements of viscoelastic properties of adherent alveolar epithelial cells by two micromanipulation techniques: (i) magnetic twisting cytometry and (ii) optical tweezers, using microbeads of same size and similarly attached to F-actin. The values of equivalent Young modulus E, derived from linear viscoelasticity theory, become consistent when the degree of bead immersion in the cell is taken into account. E-values are smaller in (i) than in (ii): similar to34-58 Pa vs similar to29-258 Pa, probably because higher stress in (i) reinforces nonlinearity and cellular plasticity. Otherwise, similar relaxation tune constants, around 2 s, suggest similar dissipative mechanisms.

引用

页码：408 / 421

页数：14

共 46 条

[31] DYNAMIC MICROMECHANICAL PROPERTIES OF CULTURED RAT ATRIAL MYOCYTES MEASURED BY ATOMIC-FORCE MICROSCOPY [J].