Ganglioside expression in tissues of mice lacking β2-microglobulin

被引:6
作者
Markotic, A
Marusic, A
Tomac, J
Müthing, J
机构
[1] Split Univ, Sch Med, Dept Biochem, Split 21000, Croatia
[2] Univ Zagreb, Sch Med, Inst Brain Res, Zagreb 41001, Croatia
[3] Univ Zagreb, Sch Med, Dept Anat, Zagreb 41001, Croatia
[4] Rijeka Univ, Sch Med, Dept Histol, Rijeka, Croatia
[5] Univ Munster, Inst Med Phys & Biophys, D-4400 Munster, Germany
关键词
gangliosides; antibodies; TLC immunostaining; gene knockout mice; beta(2)-microglobulin; MHC class I;
D O I
10.1046/j.1365-2249.2002.01802.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This study presents a comparative analysis of gangliosides from lymphoid (spleen and thymus) and other (brain, liver, lungs and muscle) tissues of C57BL/6 mice lacking the gene for B-2-microglobulin (beta(2)M), a constitutive component of the MHC class I molecule. Ganglioside fractions in the tissues of mice homozygous (B2M-/-) and heterozygous (B2M-/+) for the gene deletion were determined by high performance thin-layer chromatography (HPTLC), followed by immunostaining with specific polyclonal antibodies. Ubiquitous gangliosides G(M3)(Neu5Ac) and G(M3)(Neu5Gc) were the dominant gangliosides in the lungs of the control B2M-/+ mice, whereas the homozygous knockout mice had substantially decreased expression of these structures. The lungs of the B2M-/- mice also had reduced expression of T-lymphocyte-specific G(M1b)-type gangliosides (G(M1b) and GalNAc-G(M1b)). beta(2)M-deficient mice also had more G(M1a) and G(D1a) gangliosides in the liver, and several neolacto-series gangliosides were increased in the brain and lungs. This study provides in vivo evidence that the beta(2)M molecule can influence the acquisition of a distinct ganglioside assembly in different mouse organs, implicating its non-immunological functions.
引用
收藏
页码:27 / 35
页数:9
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