Optical detection and charge-state analysis of MALDI-generated particles with molecular masses larger than 5 MDa

被引：32

作者：

Cai, Y ^{[1
]}

Peng, WP ^{[1
]}

Kuo, SJ ^{[1
]}

Sabu, S ^{[1
]}

Han, CC ^{[1
]}

Chang, HC ^{[1
]}

机构：

[1] Acad Sinica, Inst Atom & Mol Sci, Taipei 115, Taiwan

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 17期

关键词：

D O I：

10.1021/ac020205l

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

dCharged polystyrene nanoparticles are generated by matrix assisted laser desorption/ionization (MALDI) and detected by laser-induced fluorescence (LIF) in a quadrupole ion trap. Employing the LIF technique, observations of individual fluorescent nanospheres (27 nm in diameter and containing 180 fluorescein dye equivalents) have been achieved with an average signal-to-noise ratio of similar to 10. With the trap operating at a frequency around 5 kHz, charge state analysis of the particles reveals that the number of charges carried by the spheres is between 1 and 10. It suggests a mass-to-charge ratio (m/z) in the range of 10(5)-10(6) for the MALDI-generated particles. To effectively trap such large particles (m > 5 MDa), damping of the particles' motions by using similar to50 mTorr He buffer gas is absolutely required. Similar findings are obtained for particles with a nominal size of 1 mum in diameter, demonstrating that production of charged particles with a molecular mass as high as 10(12) Da is possible using the MALDI technique.

引用

页码：4434 / 4440

页数：7

共 41 条

[1] A charge detector for time-of-flight mass analysis of high mass ions produced by matrix-assisted laser desorption/ionization (MALDI) [J].