A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

被引：190

作者：

Forcat, Silvia ^{[2
]}

Bennett, Mark H. ^{[2
]}

Mansfield, John W. ^{[2
]}

Grant, Murray R. ^{[1
]}

机构：

[1] Univ Exeter, Sch Biosci, Exeter EX4 4QD, Devon, England

[2] Univ London Imperial Coll Sci Technol & Med, Div Biol, London SW7 2AZ, England

来源：

PLANT METHODS | 2008年 / 4卷 / 1期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1186/1746-4811-4-16

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.

引用

页数：8