Synaptotagmin restores kinetic properties of a syntaxin-associated N-type voltage sensitive calcium channel

被引：69

作者：

Wiser, O ^{[1
]}

Tobi, D ^{[1
]}

Trus, M ^{[1
]}

Atlas, D ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,DEPT BIOL CHEM,IL-91904 JERUSALEM,ISRAEL

来源：

FEBS LETTERS | 1997年 / 404卷 / 2-3期

关键词：

N-type calcium channel; transmitter release; synaptotagmin; syntaxin; SNAP-25;

D O I：

10.1016/S0014-5793(97)00130-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The voltage sensitive N-type calcium channel interacts functionally and biochemically with synaptotagmin (p65), N-type channel interaction with p65 is demonstrated in the Xenopus oocyte expression system, where p65 alters the steady state voltage inactivation of the N-channel, and fully restores the syntaxin-modified current amplitude and inactivation kinetics in a calcium dependent manner, In agreement with the functional results, GST-p65 fusion protein binds to a cytosolic region, amino acids 710-1090 of the N-type channel (N-loop(710-1090)) The results of the combined approach provide a functional and biochemical basis for proposing that p65 interaction with the N-type channel brings p65 into a close association with a syntaxin-coupled channel, In turn, calcium entry through the liberated channel initiates fusion of the primed vesicles with the cell membrane at a short distance from the site of calcium entry. (C) 1997 Federation of European Biochemical Societies.

引用

页码：203 / 207

页数：5

共 33 条

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