Reappraisal of HLA Antibody Analysis and Crossmatching in Kidney Transplantation

被引：18

作者：

Lee, P. -C. ^{[1
]}

Ozawa, M. ^{[1
]}

Hung, C. -J. ^{[1
]}

Lin, Y. -J. ^{[1
]}

Chang, S. -S. ^{[1
]}

Chou, T. -C. ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Dept Surg, Tainan 70101, Taiwan

来源：

TRANSPLANTATION PROCEEDINGS | 2009年 / 41卷 / 01期

关键词：

FLOW-CYTOMETRY BEADS; CLASS-I; SPECIFICITY; RECIPIENTS; ELISA;

D O I：

10.1016/j.transproceed.2008.10.074

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Enzyme-linked immunosorbent assay (ELISA) and flow cytometric techniques have been introduced to overcome the limited sensitivity and specificity of the CDC assay. This retrospective study used lambda antigen tray-mixed screening and Luminex HLA class I and II specificity assays to re-examine: (1) the accuracy with which detection of HLA antibody and specificity by ELISA predicts pretransplantation National Institutes of Health (NIH)/Centers for Disease Control and Prevention (CDC) crossmatch; and (2) a comparison of Luminex and ELISA methods to detect HLA antibodies. Sera from 481 patients awaiting kidney transplantation were tested using the ELISA method lambda antigen tray-mixed and using NIH-CDC to determine how well HLA antibodies detected using ELISA predicted crossmatches using CDC. Pretransplantation sera from 48 patients with follow-up data were retested using both ELISA lambda antigen tray-mixed and Luminex to compare the efficacy of the 2 methods.

引用

页码：95 / 98

页数：4

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