Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror

被引：130

作者：

Piyawattanametha, Wibool

Barretto, Robert P. J.

Ko, Tony H.

Flusberg, Benjamin A.

Cocker, Eric D.

Ra, Hyejun

Lee, Daesung

Solgaard, Olav

Schnitzer, Mark J. ^{[1
]}

机构：

[1] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA

[2] Stanford Univ, Edward L Ginzton Lab, Stanford, CA 94305 USA

来源：

OPTICS LETTERS | 2006年 / 31卷 / 13期

关键词：

D O I：

10.1364/OL.31.002018

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm X 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of similar to 16 degrees. Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz. (c) 2006 Optical Society of America.

引用

页码：2018 / 2020

页数：3

共 21 条

[1] Two-photon fluorescence endoscopy with a micro-optic scanning head [J].