Two-dimensional gel electrophoresis; better than a poke in the ICAT?

被引：80

作者：

Patton, WF ^{[1
]}

Schulenberg, B ^{[1
]}

Steinberg, TH ^{[1
]}

机构：

[1] Mol Probes Inc, Proteom Sect, Eugene, OR 97402 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(02)00333-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.

引用

页码：321 / 328

页数：8

共 54 条

[1]

Aebersold R, 2000, Ann N Y Acad Sci, V919, P33

[2]

ARNOTT D, 2002, IN PRESS MOL CELL PR

[3]

Berggren K, 2000, ELECTROPHORESIS, V21, P2509, DOI 10.1002/1522-2683(20000701)21:12<2509::AID-ELPS2509>3.0.CO

[4]

2-9

[5] 2D or not 2D [J].

Fey, SJ ;

Larsen, PM .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (01) :26-33

[6]

Fountoulakis M, 2002, ELECTROPHORESIS, V23, P311, DOI 10.1002/1522-2683(200202)23:2<311::AID-ELPS311>3.0.CO

[7]

2-0

[8]

Fountoulakis M, 2001, ELECTROPHORESIS, V22, P1747, DOI 10.1002/1522-2683(200105)22:9<1747::AID-ELPS1747>3.0.CO

[9]

2-H

[10]

Futcher B, 1999, MOL CELL BIOL, V19, P7357

← 1 2 3 4 5 6 →