New PCR method to differentiate species in the Aspergillus niger aggregate

被引：30

作者：

Accensi, F ^{[1
]}

Cano, J

Figuera, L

Abarca, ML

Cabañes, FJ

机构：

[1] Univ Autonoma Barcelona, Fac Vet, Dept Patol & Prod Anim Microbiol, E-08193 Barcelona, Spain

[2] Univ Rovira & Virgili, Fac Med & Ciencies Salut, Unitat Microbiol, E-43201 Reus, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 180卷 / 02期

关键词：

taxonomy; 5.8S rDNA; PCR; RFLP analysis; Aspergillus niger; Aspergillus tubingensis;

D O I：

10.1111/j.1574-6968.1999.tb08795.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingenis) have been sequenced. By comparison of sequences we have found Chat both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：191 / 196

页数：6

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