Development of a Biomimetic Collagen-Hydroxyapatite Scaffold for Bone Tissue Engineering Using a SBF Immersion Technique

被引:147
作者
Al-Munajjed, Amir A. [1 ,2 ]
Plunkett, Niamh A. [1 ,2 ]
Gleeson, John P. [1 ,2 ]
Weber, Tim [1 ,3 ]
Jungreuthmayer, Christian [2 ]
Levingstone, Tanya [1 ]
Hammer, Joachim [3 ]
O'Brien, Fergal J. [1 ,2 ]
机构
[1] Royal Coll Surgeons Ireland, Dept Anat, Dublin 2, Ireland
[2] Trinity Coll Dublin, Trinity Ctr Bioengn, Dublin, Ireland
[3] Univ Appl Sci Regensburg, Dept Mech Engn, Regensburg, Germany
基金
爱尔兰科学基金会;
关键词
bone; tissue engineering; hydroxy(1)lapatite; biomimetic; scaffolds; CALCIUM-PHOSPHATE COATINGS; COMPOSITE SCAFFOLDS; GAG SCAFFOLDS; PORE-SIZE; IN-VITRO; MATRIX; CELLS; DIFFERENTIATION; POROSITY; OSTEOGENESIS;
D O I
10.1002/jbm.b.31320
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The objective of this study was to develop a biomimetic, highly porous collagen-hydroxyapatite (HA) composite scaffold for bone tissue engineering (TE), combining the biological performance and the high porosity of a collagen scaffold with the high mechanical stiffness of a HA scaffold. Pure collagen scaffolds were produced using a lyophilization process and immersed in simulated body fluid (SBF) to provide a biomimetic coating. Pure collagen scaffolds served as a control. The mechanical, material, and structural properties of the scaffolds were analyzed and the biological performance of the scaffolds was evaluated by monitoring the cellular metabolic activity and cell number at 1, 2, and 7 days post seeding. The SBF-treated scaffolds exhibited a significantly increased stiffness compared to the pure collagen group (4-fold increase), while a highly interconnected structure (95%) Was retained. FTIR indicated that the SBF coating exhibited similar characteristics to pure HA. Micro-CT showed a homogeneous distribution of HA. Scanning electron microscopy also indicated a mineralization of the collagen combined with a precipitation of HA onto the collagen. The excellent biological performance of the collagen scaffolds was maintained in the collagen-HA scaffolds as demonstrated from cellular metabolic activity and total cell number. This investigation has successfully developed a biomimetic collagen-HA composite scaffold. An increase in the mechanical properties combined with an excellent biological performance in vitro was observed, indicating the high potential of the scaffold for bone TE. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 584-591, 2009
引用
收藏
页码:584 / 591
页数:8
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