Luminescent pH sensing and DNA binding properties of a novel ruthenium(II) complex

被引：71

作者：

Bai, GY

Wang, KZ

Duan, ZM

Gao, LH

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

[2] Beijing Technol & Business Univ, Sch Chem & Environm Engn, Beijing 100037, Peoples R China

[3] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2004年 / 98卷 / 06期

关键词：

ruthenium complex; DNA; luminescence;

D O I：

10.1016/j.jinorgbio.2004.02.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new Ru(II) complex, [Ru(bpy)(2)(dhipH(3))](ClO4)(2) (in which bpy - 2,2'-bipyridine, dhipH(3) = 3,4-dihydroxy-imidado[4,5-f][1,10]-phenanthroline), was synthesized and characterized, and the pH effect on the emission spectra of the complex was studied. The interaction of the complex with calf thymus DNA was investigated by UV-visible and emission spectroscopy, and viscosity measurements. The results suggest that the complex acted as a sensitive luminescent pH sensor and a strong ct-DNA intercalator with an intrinsic binding constant of (4.0 +/- 0.7) x 10(5) M-1 in buffered 50 mM NaCl. (C) 2004 Elsevier Inc. All rights reserved.

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页码：1017 / 1022

页数：6

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