Metabolite transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports

被引：16

作者：

Pallotta, ML ^{[1
]}

Fratianni, A ^{[1
]}

Passarella, S ^{[1
]}

机构：

[1] Univ Molise, Dipartimento Sci Anim Vegetali & Ambiente, I-86100 Campobasso, Italy

来源：

FEBS LETTERS | 1999年 / 462卷 / 03期

关键词：

yeast mitochondrion; carrier; fumarate; ADP; ATP; transport;

D O I：

10.1016/S0014-5793(99)01535-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria, The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (K-m, and V-max,,, values are about 27 mu M and 22 nmol min(-1) mg protein(-1) and 70 mu M and 4 nmol min(-1) mg protein(-1) respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles. (C) 1999 Federation of European Biochemical Societies.

引用

页码：313 / 316

页数：4

共 22 条

[1] Fumarate permeation in normal and acidotic rat kidney mitochondria: fumarate/malate and fumarate/aspartate translocators [J].