Molecular characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures

被引:45
作者
Sun, Wenjie [1 ]
Sierra-Alvarez, Reyes [1 ]
Fernandez, Nuria [2 ]
Sanz, Jose Luis [2 ]
Amils, Ricardo [2 ]
Legatzki, Antje [3 ]
Maier, Raina M. [3 ]
Field, Jim A. [1 ]
机构
[1] Univ Arizona, Dept Environm Chem & Engn, Tucson, AZ 85721 USA
[2] Univ Autonoma Madrid, Dept Mol Biol, Madrid, Spain
[3] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ USA
关键词
chemolithotrophic; anoxic; arsenite; oxidation; Azoarcus; Comamonadaceae; POLY-BETA-HYDROXYBUTYRATE; RIBOSOMAL-RNA ANALYSIS; SP-NOV; ETHYLBENZENE DEHYDROGENASE; ACTIVATED-SLUDGE; STRAIN EBN1; GEN; NOV; AROMATOLEUM-AROMATICUM; MICROBIAL COMMUNITY; MAXIMUM-LIKELIHOOD;
D O I
10.1111/j.1574-6941.2009.00653.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To explore the bacteria involved in the oxidation of arsenite (As(III)) under denitrifying conditions, three enrichment cultures (ECs) and one mixed culture (MC) were characterized that originated from anaerobic environmental samples. The oxidation of As(III) (0.5 mM) was dependent on NO3- addition and N-2 formation was dependent on As(III) addition. The ratio of N-2-N formed to As(III) fed approximated the expected stoichiometry of 2.5. A 16S rRNA gene clone library analysis revealed three predominant phylotypes. The first, related to the genus Azoarcus from the division Betaproteobacteria, was found in the three ECs. The other two predominant phylotypes were closely related to the genera Acidovorax and Diaphorobacter within the Comamonadaceae family of Betaproteobacteria, and one of these was present in all of the cultures examined. FISH confirmed that Azoarcus accounted for a large fraction of bacteria present in the ECs. The Azoarcus clones had 96% sequence homology with Azoarcus sp. strain DAO1, an isolate previously reported to oxidize As(III) with nitrate. FISH analysis also confirmed that Comamonadaceae were present in all cultures. Pure cultures of Azoarcus and Diaphorobacter were isolated and shown to be responsible for nitrate-dependent As(III) oxidation. These results, taken as a whole, suggest that bacteria within the genus Azoarcus and the family Comamonadaceae are involved in the observed anoxic oxidation of As(III).
引用
收藏
页码:72 / 85
页数:14
相关论文
共 65 条
[11]   Denitrification with methane as electron donor in oxygen-limited bioreactors [J].
Costa, C ;
Dijkema, C ;
Friedrich, M ;
García-Encina, P ;
Fernández-Polanco, F ;
Stams, AJM .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (06) :754-762
[12]   Dokdonella fugitiva sp nov., a Gammaproteobacterium isolated from potting soil [J].
Cunha, S ;
Tiago, I ;
Pires, AL ;
da Costa, MS ;
Verissimo, A .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2006, 29 (03) :191-196
[13]  
DEGELAU A, 1995, APPL MICROBIOL BIOT, V42, P653
[14]   Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China [J].
Fan, H. ;
Su, C. ;
Wang, Y. ;
Yao, J. ;
Zhao, K. ;
Wang, Y. ;
Wang, G. .
JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (02) :529-539
[15]   EVOLUTIONARY TREES FROM DNA-SEQUENCES - A MAXIMUM-LIKELIHOOD APPROACH [J].
FELSENSTEIN, J .
JOURNAL OF MOLECULAR EVOLUTION, 1981, 17 (06) :368-376
[16]   Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp nov., Luteimonas mephitis gen. nov., sp nov and Pseudoxanthomonas broegbernensis gen. nov., sp nov. [J].
Finkmann, W ;
Altendorf, K ;
Stackebrandt, E ;
Lipski, A .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :273-282
[17]   Investigation of an acetate-fed denitrifying microbial community by stable isotope probing, full-cycle rRNA analysis, and fluorescent in situ hybridization-microautoradiography [J].
Ginige, MP ;
Keller, J ;
Blackall, LL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8683-8691
[18]   DATING OF THE HUMAN APE SPLITTING BY A MOLECULAR CLOCK OF MITOCHONDRIAL-DNA [J].
HASEGAWA, M ;
KISHINO, H ;
YANO, TA .
JOURNAL OF MOLECULAR EVOLUTION, 1985, 22 (02) :160-174
[19]   Contrasting sorption behavior of arsenic(III) and arsenic(V) in suspensions of iron and aluminum oxyhydroxides [J].
Hering, JG ;
Dixit, S .
ADVANCES IN ARSENIC RESEARCH: INTEGRATION OF EXPERIMENTAL AND OBSERVATIONAL STUDIES AND IMPLICATIONS FOR MITIGATION, 2005, 915 :8-24
[20]   In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column [J].
Hess, A ;
Zarda, B ;
Hahn, D ;
Haner, A ;
Stax, D ;
Hohener, P ;
Zeyer, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (06) :2136-2141