Flow electrochemical determination of ascorbic acid in real samples using a glassy carbon electrode modified with a cellulose acetate film bearing 2,6-dichlorophenolindophenol

被引：82

作者：

Florou, AB ^{[1
]}

Prodromidis, MI ^{[1
]}

Karayannis, MI ^{[1
]}

Tzouwara-Karayanni, SM ^{[1
]}

机构：

[1] Univ Ioannina, Dept Chem, GR-45110 Ioannina, Greece

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 409卷 / 1-2期

关键词：

electrochemical determination of ascorbic acid; controlled porosity cellulose acetate film; beverages; pharmaceuticals; flow injection analysis; 2,6-dichlorophenolindophenol;

D O I：

10.1016/S0003-2670(00)00709-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ascorbate sensor based on a glassy carbon electrode modified with a cellulose acetate polymeric film bearing 2,6-dichlorophenolindophenol (CA/DCPI-CME) was constructed. The overall reaction obeys a catalytic regeneration mechanism (EC mechanism) and the electrochemical rate constant kf for the electrocatalytic oxidation of ascorbic acid was evaluated. The modified electrodes were mounted in a flow injection (FI) manifold, poised at +100 mV versus Ag/AgCl/3 M KCl at pH 6.5 and utilized for the determination of ascorbic acid in beverages and pharmaceuticals. Good correlation with a reference method was attained. Interferents of various molecular sizes were tested. Calibration graphs were linear over the range 0.02-1 and 0.1-6 mM ascorbic acid for CA/DCPI sensors hydrolyzed in KOH and ZnCl2 solution, respectively. The throughput was 25 samples per hour and the CV was for a 0.4 mM ascorbic acid solution 0.75 (n=14) and 1.2% (n=10) for CA/DCPI sensors hydrolyzed in KOH and ZnCl2 solution, respectively. The recovery was 92-110%. The sensors showed very good repeatability and operational stability. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 121

页数：9

共 29 条

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