Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification

被引：432

作者：

Cheng, Yongqiang ^{[1
]}

Zhang, Xian ^{[1
]}

Li, Zhengping ^{[1
]}

Jiao, Xiaoxia ^{[1
]}

Wang, Yucong ^{[1
]}

Zhang, Yali ^{[1
]}

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Med Chem & Mol Diag Minist Educ, Baoding 071002, Hebei Province, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 18期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

fluorescence determination; microRNA; rolling-circle amplification; PADLOCK PROBES; SIGNAL AMPLIFICATION; AMPLIFIED SYNTHESIS; GENE-EXPRESSION; DNA DETECTION; RNA LIGASE-2; MICROARRAY; LIGATION; QUANTITATION; SPECIFICITY;

D O I：

10.1002/anie.200805665

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rollingcircle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：3268 / 3272

页数：5

相关论文

共 38 条

[1] Quantitation of microRNAs using a modified Invader assay [J].

Allawi, HT ;

Dahlberg, JE ;

Olson, S ;

Lund, E ;

Olson, M ;

Ma, WP ;

Takova, T ;

Neri, BP ;

Lyamichev, VI .

RNA, 2004, 10 (07) :1153-1161

[2] MicroRNAs - Future drug targets.? [J].

Arenz, Christoph .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (31) :5048-5050

[3] Probing microRNAs with microarrays: Tissue specificity and functional inference [J].

Babak, T ;

Zhang, W ;

Morris, Q ;

Blencowe, BJ ;

Hughes, TR .

RNA, 2004, 10 (11) :1813-1819

[4]

Cheglakov Z., 2008, ANGEW CHEM, V120, P132

[5] Nanostructures - Increasing the complexity of periodic protein nanostructures by the rolling-circle-amplified synthesis of aptamers [J].

Cheglakov, Zoya ;

Weizmann, Yossi ;

Braunschweig, Adam B. ;

Wilner, Ofer L. ;

Willner, Itamar .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (01) :126-130

[6] Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes [J].

Cheglakov, Zoya ;

Weizmann, Yossi ;

Basnar, Bernhard ;

Willner, Itamar .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (02) :223-225

[7] Real-time quantification of microRNAs by stem-loop RT-PCR [J].

Chen, CF ;

Ridzon, DA ;

Broomer, AJ ;

Zhou, ZH ;

Lee, DH ;

Nguyen, JT ;

Barbisin, M ;

Xu, NL ;

Mahuvakar, VR ;

Andersen, MR ;

Lao, KQ ;

Livak, KJ ;

Guegler, KJ .

NUCLEIC ACIDS RESEARCH, 2005, 33 (20) :e179.1-e179.9

[8] Homogeneous and label-free bioluminescence detection of single nucleotide polymorphism with rolling circle amplification [J].

Cheng, Yongqiang ;

Li, Zhengping ;

Du, Baoan ;

Zhang, Xian .

ANALYST, 2008, 133 (06) :750-752

[9] Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells [J].

Christian, AT ;

Pattee, MS ;

Attix, CM ;

Reed, BE ;

Sorensen, KJ ;

Tucker, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (25) :14238-14243

[10]

Cissell K. A., 2007, Anal. Chem, V79, P4754, DOI DOI 10.1021/AC0719305

← 1 2 3 4 →