P2X(4): An ATP-activated ionotropic receptor cloned from rat brain

被引:312
作者
Soto, F
GarciaGuzman, M
GomezHernandez, JM
Hollmann, M
Karschin, C
Stuhmer, W
机构
[1] MAX PLANCK INST EXPTL MED, DEPT MOL BIOL NEURONAL SIGNALS, D-37075 GOTTINGEN, GERMANY
[2] MAX PLANCK INST EXPTL MED, GLUTAMATE RECEPTOR LAB, D-37075 GOTTINGEN, GERMANY
关键词
purinergic receptor;
D O I
10.1073/pnas.93.8.3684
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Extracellular ATP exerts pronounced biological actions in virtually every organ or tissue that has been studied, In the central and peripheral nervous system, ATP acts as a fast excitatory transmitter in certain synaptic pathways [Evans, R. J., Derkach, V. & Surprenant, A. (1992) Nature (London) 357, 503-505; Edwards, F. A., Gigg, A. J. & Colquhoun, D. (1992) Nature (London) 359, 144-147], Here, we report the cloning and characterization of complementary DNA from rat brain, encoding an additional member (P2X(4)) of the emerging multigenic family of ligand-gated ATP channels, the P2X receptors. Expression in Xenopus oocytes gives an ATP activated cation-selective channel that is highly permeable to Ca2+ and whose sensitivity is modulated by extracellular Zn2+, Surprisingly, the current elicited by ATP is almost insensitive to the common P2X antagonist suramin, In situ hybridization reveals the expression of P2X(4) mRNA in central nervous system neurons, Northern blot and reverse transcription-PCR (RT-PCR) analysis demonstrate a wide distribution of P2X(4) transcripts in various tissues, including blood vessels and leukocytes. This suggests that the P2X(4) receptor might mediate not only ATP-dependent synaptic transmission in the central nervous system but also a wide repertoire of biological responses in diverse tissues.
引用
收藏
页码:3684 / 3688
页数:5
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